UCP-2 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20751R-BF488 |
| Product Name | UCP-2 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UCP-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7351 |
| Swiss Prot | P55851 |
| Subcellular location | Cytoplasm |
| Synonyms | Uncoupling Protein-2; UCP2; UCP 2; BMIQ4; Mitochondrial uncoupling protein 2; SLC25A8; UCPH; Uncoupling protein 2; Uncoupling protein 2 mitochondrial proton carrier; Solute carrier family 25 member 8; UCP2_HUMAN.,, |
| Background | UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is UCP-2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20751R-BF488) validated for, and at what dilution?
bs-20751R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UCP-2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20751R-BF488) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-20751R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UCP-2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-20751R-BF488)?
KLH conjugated synthetic peptide derived from human UCP-2
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-20751R-BF488)?
AbBy Fluor-488 on bs-20751R-BF488: excitation / emission 499nm/519nm.