Help

UCP-2 Polyclonal Antibody, PE-Cy5.5 Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-20751R-PE-Cy5.5
Product Name UCP-2 Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
WB
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation PE-Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human UCP-2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7351
Swiss Prot P55851
Subcellular location Cytoplasm
Synonyms Uncoupling Protein-2; UCP2; UCP 2; BMIQ4; Mitochondrial uncoupling protein 2; SLC25A8; UCPH; Uncoupling protein 2; Uncoupling protein 2 mitochondrial proton carrier; Solute carrier family 25 member 8; UCP2_HUMAN.,,
Background UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. UCP2 gene is expressed in many tissues, with the greatest expression in skeletal muscle. UCP2 is thought to play a role in non shivering thermogenesis, obesity and diabetes.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is UCP-2 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-20751R-PE-CY5-5) validated for, and at what dilution?

BS-20751R-PE-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does UCP-2 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-20751R-PE-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Rabbit.

How should BS-20751R-PE-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise UCP-2 Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-20751R-PE-CY5-5)?

KLH conjugated synthetic peptide derived from human UCP-2

What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-20751R-PE-CY5-5)?

PE-Cy5.5 on BS-20751R-PE-CY5-5: excitation / emission 488nm/694nm.