JNK1 + JNK3 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20759R-100uL |
| Product Name | JNK1 + JNK3 Polyclonal Antibody |
| Applications |
WB
IHC-P
IHC-F
IF
FLOW-CYT
ICC/IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human JNK1 + JNK3 |
| Immunogen Range | 211-300/427 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 5599 |
| Swiss Prot | P45983 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | JNK1 + JNK3; JNK1 + 3; JNK1+JNK3; JNK1/3; c Jun N terminal kinase 1; JNK1; JNK3; JAK 1A; JAK1A; JNK 1; JNK 46; JNK; JNK1A2; JNK21B1/2; MAPK 8; MAPK8; Mitogen activated protein kinase 8; PRKM 8; PRKM8; Protein kinase JNK1; SAPK 1; SAPK gamma; SAPK1; c-Jun; Stress activated protein kinase JNK1; Tyrosine protein kinase JAK1; MK08_HUMAN. |
| Background | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. |
| Application Dilution | |
| WB | ELISA1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF | IHC-P1:50-200 |
| Flow-Cyt | IFIHC-F1:50-200 |
| ICC/IF | IFICC1:50-200 |
Frequently asked questions
Which applications is JNK1 + JNK3 Polyclonal Antibody (bs-20759R) validated for, and at what dilution?
bs-20759R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does JNK1 + JNK3 Polyclonal Antibody (bs-20759R) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.
How should bs-20759R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise JNK1 + JNK3 Polyclonal Antibody (bs-20759R)?
KLH conjugated synthetic peptide derived from human JNK1 + JNK3 Immunogen range: 211-300/427.