Heme oxygenase 1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2075R-Cy3 |
| Product Name | Heme oxygenase 1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Pig |
| Predicted Reactivity | Dog, Cow, Sheep, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Heme Oxygenase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3162 |
| Swiss Prot | P09601 |
| Subcellular location | Cytoplasm |
| Synonyms | HO-1; HSP32; HMOX1D; bK286B1; HMOX1; HO; HO1 |
| Background | Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Heme oxygenase 1 Polyclonal Antibody, Cy3 Conjugated (bs-2075R-Cy3) validated for, and at what dilution?
bs-2075R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Heme oxygenase 1 Polyclonal Antibody, Cy3 Conjugated (bs-2075R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat, Pig. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Horse, Chicken, Rabbit, GuineaPig.
How should bs-2075R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Heme oxygenase 1 Polyclonal Antibody, Cy3 Conjugated (bs-2075R-Cy3)?
KLH conjugated synthetic peptide derived from human Heme Oxygenase 1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-2075R-Cy3)?
Cy3 on bs-2075R-Cy3: excitation / emission 512,550nm/570,615nm.