Heme oxygenase 1 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2075R-100uL |
| Product Name | Heme oxygenase 1 Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Pig |
| Predicted Reactivity | Dog, Cow, Sheep, Horse, Chicken, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Heme Oxygenase 1 |
| Immunogen Range | 101-200/288 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 3162 |
| Swiss Prot | P09601 |
| Subcellular location | Cytoplasm |
| Synonyms | HO-1; HSP32; HMOX1D; bK286B1; HMOX1; HO; HO1 |
| Background | Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Heme oxygenase 1 Polyclonal Antibody (bs-2075R) validated for, and at what dilution?
bs-2075R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Heme oxygenase 1 Polyclonal Antibody (bs-2075R) react with?
Tested reactivity: Human, Mouse, Rat, Pig. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Horse, Chicken, Rabbit, GuineaPig.
How should bs-2075R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Heme oxygenase 1 Polyclonal Antibody (bs-2075R)?
KLH conjugated synthetic peptide derived from human Heme Oxygenase 1 Immunogen range: 101-200/288.