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Angiotensinogen Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-20778R-APC-Cy7
Product Name Angiotensinogen Polyclonal Antibody, APC-Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse Angiotensinogen
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 11606
Swiss Prot P01019
Subcellular location Secreted
Synonyms Angiotenogen; Angiotensinogen; AT; ANGT_MOUSE; Serpin A8.,,
Background Angiotensinogen protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Angiotensinogen Polyclonal Antibody, APC-Cy7 Conjugated (bs-20778R-APC-Cy7) validated for, and at what dilution?

bs-20778R-APC-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Angiotensinogen Polyclonal Antibody, APC-Cy7 Conjugated (bs-20778R-APC-Cy7) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-20778R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Angiotensinogen Polyclonal Antibody, APC-Cy7 Conjugated (bs-20778R-APC-Cy7)?

KLH conjugated synthetic peptide derived from mouse Angiotensinogen

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-20778R-APC-Cy7)?

APC-Cy7 on bs-20778R-APC-Cy7: excitation / emission 650nm/780nm.