Angiotensinogen Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20778R-Cy3-100uL |
| Product Name | Angiotensinogen Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Angiotensinogen |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 11606 |
| Swiss Prot | P01019 |
| Subcellular location | Secreted |
| Synonyms | Angiotenogen; Angiotensinogen; AT; ANGT_MOUSE; Serpin A8.,, |
| Background | Angiotensinogen protein encoded by this gene, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Angiotensinogen Polyclonal Antibody, Cy3 Conjugated (bs-20778R-Cy3) validated for, and at what dilution?
bs-20778R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Angiotensinogen Polyclonal Antibody, Cy3 Conjugated (bs-20778R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-20778R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Angiotensinogen Polyclonal Antibody, Cy3 Conjugated (bs-20778R-Cy3)?
KLH conjugated synthetic peptide derived from mouse Angiotensinogen
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-20778R-Cy3)?
Cy3 on bs-20778R-Cy3: excitation / emission 512,550nm/570,615nm.