CD19 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20781R-BF350 |
| Product Name | CD19 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
FCM
IF(ICC)
IF
|
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD19 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 930 |
| Swiss Prot | P15391 |
| Synonyms | Antibody deficiency due to defect in CD19, included; AW495831; B lymphocyte antigen CD19; B lymphocyte surface antigen B4; B4; CD 19; CD19 antigen; CD19 molecule; Cd19 protein; Differentiation Antigen CD19; Leu 12; Leu12; Lymphocyte Surface Antigen; MGC109570; MGC12802; T-cell surface antigen Leu-12. |
| Background | CD19 is a transmembrane glycoprotein that is delectively expressed on the cell surface of B-lymphocytes,where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways.Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. |
| Application Dilution | |
| WB | |
| FCM | 1:20-100 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is CD19 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-20781R-BF350) validated for, and at what dilution?
bs-20781R-BF350 is validated for WB; FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD19 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-20781R-BF350) react with?
Tested reactivity: Human.
How should bs-20781R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD19 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-20781R-BF350)?
KLH conjugated synthetic peptide derived from human CD19
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-20781R-BF350)?
AbBy Fluor-350 on bs-20781R-BF350: excitation / emission 346nm/442nm.