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CD19 Polyclonal Antibody, FITC Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-20782R-FITC
Product Name CD19 Polyclonal Antibody, FITC Conjugated
Applications
WB FCM
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Sheep, Pig, Horse, Rabbit
Specifications
Conjugation FITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD19
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 930
Swiss Prot P15391
Subcellular location Cell membrane
Synonyms Antibody deficiency due to defect in CD19, included; AW495831; B lymphocyte antigen CD19; B lymphocyte surface antigen B4; B4; CD 19; CD19 antigen; CD19 molecule; Cd19 protein; Differentiation Antigen CD19; Leu 12; Leu12; Lymphocyte Surface Antigen; MGC109570; MGC12802; T-cell surface antigen Leu-12
Background Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is CD19 Polyclonal Antibody, FITC Conjugated (bs-20782R-FITC) validated for, and at what dilution?

bs-20782R-FITC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD19 Polyclonal Antibody, FITC Conjugated (bs-20782R-FITC) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Pig, Horse, Rabbit.

How should bs-20782R-FITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD19 Polyclonal Antibody, FITC Conjugated (bs-20782R-FITC)?

KLH conjugated synthetic peptide derived from human CD19

What are the excitation and emission wavelengths of the FITC conjugate (bs-20782R-FITC)?

FITC on bs-20782R-FITC: excitation / emission 494nm/518nm.