DCX/Doublecortin Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-20797R-Cy5-100uL |
| Product Name | DCX/Doublecortin Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DCX/Doublecortin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1641 |
| Swiss Prot | O43602 |
| Synonyms | Doublecortex; DBCN; Dbct; DC; Doublin; Lis X; Lissencephalin X; Lissencephaly X linked; Lissencephaly X linked doublecortin; LISX; Neuronal migration protein doublecortin; SCLH; XLIS. |
| Background | Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. Neuronal precursors begin to express DCX shortly after exiting the cell cycle, and continue to express DCX for 2-3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express, a marker for mature neurons. Due to the nearly exclusive expression of DCX in developing neurons, this protein has been used increasingly as a marker for neurogenesis. Indeed, the levels of DCX expression increase in response to exercise, which occurs in parallel with increased BrdU labelling, currently a "gold standard" in measuring neurogenesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | |
Frequently asked questions
Which applications is DCX/Doublecortin Polyclonal Antibody, Cy5 Conjugated (bs-20797R-Cy5) validated for, and at what dilution?
bs-20797R-Cy5 is validated for WB (1:300-5000); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DCX/Doublecortin Polyclonal Antibody, Cy5 Conjugated (bs-20797R-Cy5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Rabbit.
How should bs-20797R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DCX/Doublecortin Polyclonal Antibody, Cy5 Conjugated (bs-20797R-Cy5)?
KLH conjugated synthetic peptide derived from human DCX/Doublecortin
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-20797R-Cy5)?
Cy5 on bs-20797R-Cy5: excitation / emission 625,650nm/670nm.