DCX/Doublecortin Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-20798r-bf750-100uL |
| Product Name | DCX/Doublecortin Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(ICC)
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DCX |
| Immunogen Range | 311-400/441 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1641 |
| Swiss Prot | O43602 |
| Synonyms | Doublecortex; DBCN; Dbct; DC; Doublin; Lis X; Lissencephalin X; Lissencephaly X linked; Lissencephaly X linked doublecortin; LISX; Neuronal migration protein doublecortin; SCLH; XLIS. |
| Background | Doublecortin (DCX) is a microtubule-associated protein expressed almost exclusively in immature neurons. Neuronal precursors begin to express DCX shortly after exiting the cell cycle, and continue to express DCX for 2-3 weeks as the cells mature into neurons. Downregulation of DCX begins after 2 weeks, and occurs at the same time that these cells begin to express, a marker for mature neurons. Due to the nearly exclusive expression of DCX in developing neurons, this protein has been used increasingly as a marker for neurogenesis. Indeed, the levels of DCX expression increase in response to exercise, which occurs in parallel with increased BrdU labelling, currently a "gold standard" in measuring neurogenesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(ICC) | 1:50-200 |
| IF | 1:50-200 |
Frequently asked questions
Which applications is DCX/Doublecortin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-20798R-BF750) validated for, and at what dilution?
bs-20798R-BF750 is validated for WB (1:300-5000); IF(ICC) (1:50-200); IF (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DCX/Doublecortin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-20798R-BF750) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-20798R-BF750 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DCX/Doublecortin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-20798R-BF750)?
KLH conjugated synthetic peptide derived from human DCX Immunogen range: 311-400/441.
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-20798R-BF750)?
AbBy Fluor-750 on bs-20798R-BF750: excitation / emission 749nm/775nm.