phospho-Caldesmon (Ser789) Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2082R-BF680 |
| Product Name | phospho-Caldesmon (Ser789) Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Caldesmon around the phosphorylation site of Ser789 |
| Modification Site | Ser789 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 800 |
| Subcellular location | Cytoplasm |
| Synonyms | Caldesmon S789; Caldesmon Ser789; p-Caldesmon S789; p-Caldesmon Ser789; p-Caldesmon Ser789; CAD; CALD 1; CALD1; CALD1_HUMAN; Caldesmon 1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; Caldesmon; Caldesmon1; CDM; H CAD; HCAD; L CAD; LCAD; MGC21352; NAG22. |
| Background | This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Caldesmon (Ser789) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2082R-BF680) validated for, and at what dilution?
bs-2082R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Caldesmon (Ser789) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2082R-BF680) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Horse, Rabbit.
How should bs-2082R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Caldesmon (Ser789) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2082R-BF680)?
KLH conjugated synthetic phosphopeptide derived from human Caldesmon around the phosphorylation site of Ser789
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-2082R-BF680)?
AbBy Fluor-680 on bs-2082R-BF680: excitation / emission 679nm/702nm.