TEM1 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2101R-PE-Cy5 |
| Product Name | TEM1 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TEM 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 57124 |
| Swiss Prot | Q9HCU0 |
| Subcellular location | Cell membrane |
| Synonyms | TEM1; CD164L1; Endosialin; Tumor endothelial marker 1; CD248 |
| Background | The transmembrane glycoprotein Tumor endothelial marker 1 (TEM1) is highly expressed in tumor endothelial cells however it is barely detectable on normal endothelial cells. It is believed to play a role in tumor angiogenesis. It is expressed in stromal fibroblasts, metastatic hepatic lesions and during angiogenesis of corpus luteum formation and wound healing. TEM1 is being investigated as a potential target for cancer treatment. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is TEM1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-2101R-PE-Cy5) validated for, and at what dilution?
bs-2101R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TEM1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-2101R-PE-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse.
How should bs-2101R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TEM1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-2101R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human TEM 1
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-2101R-PE-Cy5)?
PE-Cy5 on bs-2101R-PE-Cy5: excitation / emission 488nm/670nm.