POU6F1/BRN5 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-21049R |
| Product Name | POU6F1/BRN5 Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human POU6F1/BRN5 |
| Immunogen Range | 25-120/301 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 5463 |
| Swiss Prot | Q14863 |
| Subcellular location | Nucleus |
| Synonyms | Brain 5; Brain specific homeobox/POU domain protein 5; Brn 5; Brn 5 protein; BRN5; Emb; MPOU; mPOU homeobox protein; POU class 6 homeobox 1; POU domain class 6 transcription factor 1; TCFB1. |
| Background | The POU6F1 gene encodes a protein that is part of a family of transcription factors which exhibit distinct temporal and spatial patterns of expression. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is POU6F1/BRN5 Polyclonal Antibody (bs-21049R) validated for, and at what dilution?
bs-21049R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does POU6F1/BRN5 Polyclonal Antibody (bs-21049R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-21049R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise POU6F1/BRN5 Polyclonal Antibody (bs-21049R)?
KLH conjugated synthetic peptide derived from human POU6F1/BRN5 Immunogen range: 25-120/301.