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PXMP4 Polyclonal Antibody, Cy5 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Chicken
  • Zebrafish
Overview
Catalog # bs-21115R-Cy5-100uL
Product Name PXMP4 Polyclonal Antibody, Cy5 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Chicken, Zebrafish
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human PXMP4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 11264
Swiss Prot Q9Y6I8
Subcellular location Cytoplasm
Synonyms 24 kDa peroxisomal intrinsic membrane protein; Peroxisomal membrane protein 4 (24kD); Peroxisomal membrane protein 4; Peroxisomal membrane protein 4, 24kDa; PMP24; PXMP4_HUMAN.
Background PXMP4 belongs to the peroxisomal membrane protein PXMP2/4 family. Hypermethylation-mediated silencing of PXMP4 may be involved in prostate carcinogenesis. PXMP4 undergoes hypermethylation and silencing during the transition of prostate adenocarcinoma cells from androgen dependence to androgen independence.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is PXMP4 Polyclonal Antibody, Cy5 Conjugated (bs-21115R-Cy5) validated for, and at what dilution?

bs-21115R-Cy5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does PXMP4 Polyclonal Antibody, Cy5 Conjugated (bs-21115R-Cy5) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Chicken, Zebrafish.

How should bs-21115R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise PXMP4 Polyclonal Antibody, Cy5 Conjugated (bs-21115R-Cy5)?

KLH conjugated synthetic peptide derived from human PXMP4

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-21115R-Cy5)?

Cy5 on bs-21115R-Cy5: excitation / emission 625,650nm/670nm.