SLC25A23 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SLC25A23) Polyclonal Antibody, Unconjugated (bs-21225R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (SLC25A23) Polyclonal Antibody, Unconjugated (bs-21225R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with SLC25A23 Polyclonal Antibody, Unconjugated (bs-21225R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.$

Lane 1: Mouse Cerebrum tissue lysates; Lane 2: Rat Cerebrum tissue lysates probed with SLC25A23 Polyclonal Antibody, Unconjugated (bs-21225R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Pig
Horse

Overview
Catalog #	bs-21225r
Product Name	SLC25A23 Polyclonal Antibody
Applications	WB IHC-P IHC-F IF
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Pig, Horse
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human SLC25A23
Clonality	Polyclonal
Isotype	IgG
Concentration	1mg/ml
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	79085
Swiss Prot	Q9BV35
Synonyms	APC2; Calcium binding mitochondrial carrier protein SCaMC3; FLJ30339; MCSC2; MGC2615; Mitochondrial ATP Mg/Pi carrier protein 2; Mitochondrial Ca(2+) dependent solute carrier protein 2; SCAMC3; Small calcium binding mitochondrial carrier protein 3; Solute carrier family 25 member 23;
Application Dilution
WB	=1:500-2000
IHC-P	IHC-P=1:100-500
IHC-F	IHC-F=1:100-500
IF	IF=1:100-500