GST tag Polyclonal Antibody

Lane 1: GST-Tagged Fusion Protein Overexpression E.coli lysates; Lane 2: GST-Tagged Fusion Protein Overexpression E.coli lysates probed with GST tag Polyclonal Antibody, Unconjugated (bs-2122R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: GST-tagged fusion protein probed with GST tag Polyclonal Antibody, Unconjugated (bs-2122R) at 1:60000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bs-2122R (Anti-GST) at dilution of 1:60,000 incubated at 4\u2103 overnight.$

Transformed (GST-tag or EV) E. coli cells lysates were subjected to SDS-PAGE followed by WB with bs-2122R (Anti-GST) at dilution of 1:60,000 incubated at 4\u2103 overnight.

Applications

WB
ELISA

Reactivity

Species independent

Overview
Catalog #	bs-2122R
Product Name	GST tag Polyclonal Antibody
Applications	WB, ELISA
Reactivity	Species independent
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	Recombinant full length GST tag protein
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Subcellular location	Cytoplasm
Synonyms	Glutathione S Transferase; Glutathione S-transferase class-mu 26 kDa isozyme; GST; Sj26 antigen; SjGST; GST-tag.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000