Receive 30% off all Monoclonal IHC antibodies with code "MONO-IHC-30"! Offer valid for US customers and available internationally through distributors.

Contact us at 1.800.501.7654 or info@biossusa.com

MenuSearch

Cyp2J3 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse heart labeled with Anti-Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse heart labeled with Anti-Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: rat liver lysates Lane 2: rat brain lysates probed with Anti Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD.
Lane 1: rat liver lysates Lane 2: rat brain lysates probed with Anti Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 55kD. Observed band size: 55kD.
Lane 1: Liver lysates; Lane 2: Brain lysates probed with Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Lane 1: Liver lysates; Lane 2: Brain lysates probed with Cyp2-j3 Polyclonal Antibody, Unconjugated (bs-2123R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-2123R
Product Name Cyp2J3 Polyclonal Antibody
Applications WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity Mouse
Predicted Reactivity Rat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Cyp2-j3
Clonality Polyclonal
Clone # #REF!
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1573
Swiss Prot P51589
Subcellular location Cytoplasm, Cell membrane
Synonyms CPJ2; Cytochrome P450 2J2; Arachidonic acid epoxygenase; CYPIIJ2; CYP2J2
Background This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Search our catalog of hundreds of thousands of products