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5HT4 Receptor Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Chicken
Overview
Catalog # bs-2127R-PE
Product Name 5HT4 Receptor Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Chicken
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human 5-HTR4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3360
Swiss Prot Q13639
Subcellular location Cytoplasm, Cell membrane
Synonyms 5-HT4; 5-HT4R; 5-hydroxytryptamine receptor 4; 5-HT-4; Serotonin receptor 4; HTR4
Background This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is 5HT4 Receptor Polyclonal Antibody, PE Conjugated (bs-2127R-PE) validated for, and at what dilution?

bs-2127R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does 5HT4 Receptor Polyclonal Antibody, PE Conjugated (bs-2127R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Chicken.

How should bs-2127R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise 5HT4 Receptor Polyclonal Antibody, PE Conjugated (bs-2127R-PE)?

KLH conjugated synthetic peptide derived from human 5-HTR4

What are the excitation and emission wavelengths of the PE conjugate (bs-2127R-PE)?

PE on bs-2127R-PE: excitation / emission 496,564nm/578nm.