OST-beta Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2128R-100uL |
| Product Name | OST-beta Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human OST-beta |
| Immunogen Range | 1-35/128 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 123264 |
| Subcellular location | Cell membrane |
| Synonyms | organic solute transporter beta Ostbeta; Organic solute transporter subunit beta; Ostbeta; Ost beta; OST-beta; OSTB; OSTB_HUMAN. |
| Background | The heteromeric transporter OST Alpha/OST Beta facilitates the transport of bile and other steroid solutes across the basolateral epithelial cell membrane of intestine, liver, testis, kidney and adrenal gland. OST Alpha/OST Beta expression is induced by bile acids through ligand-dependent transactivation of their genes by FXR (Farnesoid X-activated receptor). This genetic regulation suggests that in response to changes in intracellular bile acid levels, bile acids adjust the rate of their own efflux from enterocytes. OST Beta is a 128 amino acid single-pass transmembrane protein that requires OST Alpha to localize to the plasma membrane. Coexpression of OST Alpha and OST Beta is also required to convert the OST Alpha subunit to a mature glycosylated endoglycosidase H-resistant form, suggesting that co-expression facilitates trafficking of OST Alpha through the golgi apparatus. Though widely expressed, OST Beta is present at highest levels in ileum. |
| Application Dilution | |
| ELISA | WB1:300-5000 |
| IHC-P | ELISA1:500-1000 |
| IHC-F | IHC-P1:200-400 |
| IF | |
Frequently asked questions
Which applications is OST-beta Polyclonal Antibody (bs-2128R) validated for, and at what dilution?
bs-2128R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does OST-beta Polyclonal Antibody (bs-2128R) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-2128R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise OST-beta Polyclonal Antibody (bs-2128R)?
KLH conjugated synthetic peptide derived from human OST-beta Immunogen range: 1-35/128.