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RSPO1 Polyclonal Antibody, Cy5 Conjugated

Applications

  • IF

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Dog
  • Pig
  • Rabbit
  • Horse
Overview
Catalog # bs-21385R-Cy5
Product Name RSPO1 Polyclonal Antibody, Cy5 Conjugated
Applications
IF
Reactivity Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Horse
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human RSPO1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 284654
Swiss Prot Q2MKA7
Subcellular location Secreted
Synonyms CRISTIN3; FLJ40906; hRspo1; R spondin homolog (Xenopus laevis); R spondin homolog; R spondin1; R-spondin-1; Roof plate specific spondin; Roof plate-specific spondin-1; RP11-566C13.1; RSPO; Rspo1; RSPO1_HUMAN.
Background RSPO1 is a member of the R-spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects.
Application Dilution
IF ICCICC1:50-200

Frequently asked questions

Which applications is RSPO1 Polyclonal Antibody, Cy5 Conjugated (bs-21385R-Cy5) validated for, and at what dilution?

bs-21385R-Cy5 is validated for IF(ICC)(IF(ICC)) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does RSPO1 Polyclonal Antibody, Cy5 Conjugated (bs-21385R-Cy5) react with?

Tested reactivity: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Horse.

How should bs-21385R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise RSPO1 Polyclonal Antibody, Cy5 Conjugated (bs-21385R-Cy5)?

KLH conjugated synthetic peptide derived from human RSPO1

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-21385R-Cy5)?

Cy5 on bs-21385R-Cy5: excitation / emission 625,650nm/670nm.