Klrb1 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-21419R-Cy3 |
| Product Name | Klrb1 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat Klrb1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 362443 |
| Swiss Prot | Q0ZUP0 |
| Subcellular location | Cell membrane |
| Synonyms | Killer cell lectin-like receptor subfamily B member 1; Killer cell lectin-like receptor subfamily B member 1G; Natural killer cell surface protein NKR-P1G; Natural killer lectin-like receptor 1E; Gm4696; Klrb1d; Klrb1g; Klrb6; Nkrp1g; Ly55; Ly-55; NKR-P1G; Nkrp-1e; 4930431A04Rik; KLRB1_MOUSE; KLRB1_RAT; CD161c(mouse); CD161a(rat); |
| Background | Plays an inhibitory role on natural killer (NK) cells cytotoxicity. Activation results in specific acid sphingomyelinase/SMPD1 stimulation with subsequent marked elevation of intracellular ceramide. Activation also leads to AKT1/PKB and RPS6KA1/RSK1 kinases stimulation as well as markedly enhanced T-cell proliferation induced by anti-CD3. Acts as a lectin that binds to the terminal carbohydrate Gal-alpha(1,3)Gal epitope as well as to the N-acetyllactosamine epitope. Binds also to CLEC2D/LLT1 as a ligand and inhibits NK cell-mediated cytotoxicity as well as interferon-gamma secretion in target cells. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Klrb1 Polyclonal Antibody, Cy3 Conjugated (bs-21419R-Cy3) validated for, and at what dilution?
bs-21419R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Klrb1 Polyclonal Antibody, Cy3 Conjugated (bs-21419R-Cy3) react with?
Tested reactivity: Mouse, Rat.
How should bs-21419R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Klrb1 Polyclonal Antibody, Cy3 Conjugated (bs-21419R-Cy3)?
KLH conjugated synthetic peptide derived from rat Klrb1
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-21419R-Cy3)?
Cy3 on bs-21419R-Cy3: excitation / emission 512,550nm/570,615nm.