E2F3 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-21438R-BF680 |
| Product Name | E2F3 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Dog, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human E2F3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1871 |
| Swiss Prot | O00716 |
| Subcellular location | Nucleus |
| Synonyms | DKFZp686C18211; E2F 3; E2F transcription factor 3; KIAA0075; MGC104598; Transcription factor E2F 3; E2F3_HUMAN; Transcription factor E2F3; E2F-3. |
| Background | The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner. [provided by RefSeq]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is E2F3 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-21438R-BF680) validated for, and at what dilution?
bs-21438R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does E2F3 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-21438R-BF680) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Horse, Rabbit.
How should bs-21438R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise E2F3 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-21438R-BF680)?
KLH conjugated synthetic peptide derived from human E2F3
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-21438R-BF680)?
AbBy Fluor-680 on bs-21438R-BF680: excitation / emission 679nm/702nm.