Hepatitis B virus pre-X protein Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-2147R-BF555 |
| Product Name | Hepatitis B virus pre-X protein Polyclonal Antibody, AbBy Fluor-555 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Virus, Others |
| Specifications | |
| Conjugation | AbBy Fluor-555 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Hepatitis B virus X protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | HBX; pre-X protein; HB-X; X protein; HBV X protein; X protein [Hepatitis B virus]. |
| Background | Multifunctional protein that may modulate protein degradation pathways, apoptosis, transcription, signal transduction, cell cycle progress, and genetic stability by directly or indirectly interacting with hosts factors. Does not seem to be essential for HBV infection. May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma). Most of cytosolic activities involve modulation of cytosolic calcium. The effect on apoptosis is controversial depending on the cell types in which the studies have been conducted. By binding to human DDB1, may affect cell viability and stimulate genome replication. May induce apoptosis by localizing in mitochondria and causing loss of mitochondrial membrane potential. May also modulate apoptosis by binding human CFLAR, a key regulator of the death-inducing signaling complex (DISC). Moderately stimulates transcription of many different viral and cellular transcription elements. Promoters and enhancers stimulated by HBx contain DNA binding sites for NF-kappa-B, AP-1, AP-2, c-EBP, ATF/CREB, or the calcium-activated factor NF-AT. May bind bZIP transcription factors like CREB1 (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Hepatitis B virus pre-X protein Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2147R-BF555) validated for, and at what dilution?
bs-2147R-BF555 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Hepatitis B virus pre-X protein Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2147R-BF555) react with?
Tested reactivity: Virus, Others.
How should bs-2147R-BF555 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Hepatitis B virus pre-X protein Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-2147R-BF555)?
KLH conjugated synthetic peptide derived from Hepatitis B virus X protein
What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-2147R-BF555)?
AbBy Fluor-555 on bs-2147R-BF555: excitation / emission 553nm/568nm.