Help

Ceramide glucosyltransferase Polyclonal Antibody, Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-21562R-Cy7
Product Name Ceramide glucosyltransferase Polyclonal Antibody, Cy7 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Ceramide glucosyltransferase
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7357
Swiss Prot Q16739
Subcellular location Cytoplasm, Cell membrane
Synonyms CEGT_HUMAN; UGCG; GCS; GLCT 1; GLCT-1; GLCT1; Glucosylceramide synthase; UDP glucose ceramide glucosyltransferase; UDP glucose N acylsphingosine D glucosyltransferase; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase; Ugcg.
Background Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase".
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Ceramide glucosyltransferase Polyclonal Antibody, Cy7 Conjugated (bs-21562R-Cy7) validated for, and at what dilution?

bs-21562R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Ceramide glucosyltransferase Polyclonal Antibody, Cy7 Conjugated (bs-21562R-Cy7) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Sheep, Pig, Horse, Chicken, Rabbit.

How should bs-21562R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Ceramide glucosyltransferase Polyclonal Antibody, Cy7 Conjugated (bs-21562R-Cy7)?

KLH conjugated synthetic peptide derived from human Ceramide glucosyltransferase

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-21562R-Cy7)?

Cy7 on bs-21562R-Cy7: excitation / emission 743nm/767nm.