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Brachyury Polyclonal Antibody, RBITC Conjugated

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-21569r-rbitc-100uL
Product Name Brachyury Polyclonal Antibody, RBITC Conjugated
Applications
WB
Reactivity Human, Mouse, Rat
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Brachyury
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID O15178
Swiss Prot 6862
Synonyms BRAC_HUMAN; Brachyury homolog; Brachyury protein; Bry; MGC104817; Protein T; T; T brachyury homolog; T Protein; T, brachyury homolog (mouse); TFT; Transcription factor T。
Background he protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is Brachyury Polyclonal Antibody, RBITC Conjugated (bs-21569R-RBITC) validated for, and at what dilution?

bs-21569R-RBITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Brachyury Polyclonal Antibody, RBITC Conjugated (bs-21569R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-21569R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Brachyury Polyclonal Antibody, RBITC Conjugated (bs-21569R-RBITC)?

KLH conjugated synthetic peptide derived from human Brachyury

What are the excitation and emission wavelengths of the RBITC conjugate (bs-21569R-RBITC)?

RBITC on bs-21569R-RBITC: excitation / emission 570nm/595nm.