Glypican 4 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2159R-BF350 |
| Product Name | Glypican 4 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse, Rabbit, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Glypican 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2239 |
| Swiss Prot | O75487 |
| Subcellular location | Secreted, Cell membrane, Extracellular matrix |
| Synonyms | Glypican 4; Glypican proteoglycan 4; Gpc4; K Glypican; K-glypican; Secreted glypican 4; GPC4_HUMAN. |
| Background | Glypicans are glycosylphosphatidylinositol-anchored heparan sulphate proteoglycans, which are thought to modulate signalling by growth factors, including fibroblast growth factors and Wnts. Expression of glypican-4 has been reported in the mouse brain at embryonic day 7 and later stages. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Glypican 4 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-2159R-BF350) validated for, and at what dilution?
bs-2159R-BF350 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Glypican 4 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-2159R-BF350) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, Rabbit, GuineaPig.
How should bs-2159R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Glypican 4 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-2159R-BF350)?
KLH conjugated synthetic peptide derived from human Glypican 4
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-2159R-BF350)?
AbBy Fluor-350 on bs-2159R-BF350: excitation / emission 346nm/442nm.