AHR Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-21600R-Cy3-100uL |
| Product Name | AHR Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human AHR |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 196 |
| Swiss Prot | P35869 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Ah receptor; AHR; Aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; Aryl hydrocarbon receptor precursor; BHLHE76; Class E basic helix loop helix protein 76; HGNC:348; AHR_HUMAN. |
| Background | Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Likely to play an important role in the development and maturation of many tissues. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is AHR Polyclonal Antibody, Cy3 Conjugated (bs-21600R-Cy3) validated for, and at what dilution?
bs-21600R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AHR Polyclonal Antibody, Cy3 Conjugated (bs-21600R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, Pig, Horse, Rabbit.
How should bs-21600R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AHR Polyclonal Antibody, Cy3 Conjugated (bs-21600R-Cy3)?
KLH conjugated synthetic peptide derived from human AHR
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-21600R-Cy3)?
Cy3 on bs-21600R-Cy3: excitation / emission 512,550nm/570,615nm.