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Nm23-H2/NME2 Polyclonal Antibody, AbBy Fluor-555 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Cow
  • Sheep
  • Pig
  • Chicken
  • Rabbit
Overview
Catalog # bs-21604R-BF555-100uL
Product Name Nm23-H2/NME2 Polyclonal Antibody, AbBy Fluor-555 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat, Cow, Sheep, Pig, Chicken, Rabbit
Specifications
Conjugation AbBy Fluor-555
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Nm23-H2/NME2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4831
Swiss Prot P22392
Subcellular location Cytoplasm, Nucleus
Synonyms nucleoside dIPhosphatase kinase B; C myc purine binding transcription factor PUF; NDK B; NDKB; NDP kinase B; NDPKB; NM23 H2; NM23B; Nucleoside diphosphate kinase B; nm23-M2; NME2; Nucleoside diphosphate kinase alpha, p18-12d; puf; MGC107562; MGC107563; MGC111212; non-metastatic cells 2, protein (NM23B)expressed in; NDKB_HUMAN; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB; nm23-H2.
Background Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Nm23-H2/NME2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-21604R-BF555) validated for, and at what dilution?

bs-21604R-BF555 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Nm23-H2/NME2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-21604R-BF555) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Pig, Chicken, Rabbit.

How should bs-21604R-BF555 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Nm23-H2/NME2 Polyclonal Antibody, AbBy Fluor-555 Conjugated (bs-21604R-BF555)?

KLH conjugated synthetic peptide derived from human Nm23-H2/NME2

What are the excitation and emission wavelengths of the AbBy Fluor-555 conjugate (bs-21604R-BF555)?

AbBy Fluor-555 on bs-21604R-BF555: excitation / emission 553nm/568nm.