COL4A3/Tumstatin Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2160R-Biotin-100uL |
| Product Name | COL4A3/Tumstatin Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
ELISA
IHC-P
IHC-F
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tumstatin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 1285 |
| Subcellular location | Secreted, Cell membrane, Extracellular matrix |
| Synonyms | Collagen alpha-3IV chain; Alpha 3 type IV collagen; Alpha3 type IV collagen; COL4A 3; COL4A3; Collagen IV alpha 3 polypeptide; Collagen type IV alpha 3 Goodpasture antigen; Collagen type IV alpha 3; Goodpasture antigen; OTTHUMP00000195044; Tumstatin; Col4a3; CO4A3_HUMAN. |
| Background | COL4A3 (Collagen, type IV, alpha 3) belongs to the type IV collagen family. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Type IV collagen is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes, alpha 1 through alpha 6, each of which can form a triple helix structure with 2 other subunits to form type IV collagen. Tumstatin, a cleavage fragment corresponding to the collagen alpha 3(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is COL4A3/Tumstatin Polyclonal Antibody, Biotin Conjugated (bs-2160R-Biotin) validated for, and at what dilution?
bs-2160R-Biotin is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does COL4A3/Tumstatin Polyclonal Antibody, Biotin Conjugated (bs-2160R-Biotin) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat.
How should bs-2160R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise COL4A3/Tumstatin Polyclonal Antibody, Biotin Conjugated (bs-2160R-Biotin)?
KLH conjugated synthetic peptide derived from human Tumstatin