DKK1 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2162R-BF405-100uL |
| Product Name | DKK1 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Dog |
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DKK1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22943 |
| Swiss Prot | O94907 |
| Subcellular location | Secreted |
| Synonyms | SK; DKK-1; Dickkopf-related protein 1; Dickkopf-1; hDkk-1; DKK1; UNQ492/PRO18 |
| Background | Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DKK1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-2162R-BF405) validated for, and at what dilution?
bs-2162R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does DKK1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-2162R-BF405) react with?
Tested reactivity: Dog. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Rat.
How should bs-2162R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DKK1 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-2162R-BF405)?
KLH conjugated synthetic peptide derived from human DKK1
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-2162R-BF405)?
AbBy Fluor-405 on bs-2162R-BF405: excitation / emission 401nm/421nm.