STIM1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-21725R-Cy5.5 |
| Product Name | STIM1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human STIM1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6786 |
| Swiss Prot | Q13586 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | GOK; SIM; STIM 1; STIM1; STIM-1; Stim1 stromal interaction molecule 1; STIM1_HUMAN; STIM1L; Stromal interaction molecule 1. |
| Background | Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is STIM1 Polyclonal Antibody, Cy5.5 Conjugated (BS-21725R-CY5-5) validated for, and at what dilution?
BS-21725R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does STIM1 Polyclonal Antibody, Cy5.5 Conjugated (BS-21725R-CY5-5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Sheep, Horse.
How should BS-21725R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise STIM1 Polyclonal Antibody, Cy5.5 Conjugated (BS-21725R-CY5-5)?
KLH conjugated synthetic peptide derived from human STIM1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-21725R-CY5-5)?
Cy5.5 on BS-21725R-CY5-5: excitation / emission 675nm/694nm.