Glypican 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-21788R-BF594-100uL |
| Product Name | Glypican 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Glypican 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 14733 |
| Swiss Prot | Q9QZF2 |
| Subcellular location | Cytoplasm, Secreted, Cell membrane, Extracellular matrix |
| Synonyms | FLJ38078; glypican; glypican1; GPC 1; Glypican-1; GPC1; Glypican proteoglycan 1; Gpc1; GPC1_HUMAN; Secreted glypican-1. |
| Background | Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparin sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Glypican 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-21788R-BF594) validated for, and at what dilution?
bs-21788R-BF594 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Glypican 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-21788R-BF594) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Sheep, Horse.
How should bs-21788R-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Glypican 1 Polyclonal Antibody, AbBy Fluor-594 Conjugated (bs-21788R-BF594)?
KLH conjugated synthetic peptide derived from mouse Glypican 1
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bs-21788R-BF594)?
AbBy Fluor-594 on bs-21788R-BF594: excitation / emission 590nm/617nm.