SM22 Alpha Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2178R-RBITC |
| Product Name | SM22 Alpha Polyclonal Antibody, RBITC Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SM22 Alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6876 |
| Subcellular location | Cytoplasm |
| Synonyms | 22 kDa actin binding protein; 22 kDa actin-binding protein; Transgelin; DKFZp686P11128; Human 22kDa smooth muscle protein; Protein WS3-10; SM22 alpha; SM22; SM22-alpha; SMCC; Smooth muscle protein 22-alpha; TAGL_HUMAN; TAGLN; TAGLN1; transgelin; Transgelin variant 2; WS3 10; Protein WS3-10; WS3-10. |
| Background | SM22 alpha is an actin cross-linking/gelling protein that belongs to the calponin family. It is involved in calcium interactions and contractile properties of the cell. SM22 may contribute to replicative senescence. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SM22 Alpha Polyclonal Antibody, RBITC Conjugated (bs-2178R-RBITC) validated for, and at what dilution?
bs-2178R-RBITC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SM22 Alpha Polyclonal Antibody, RBITC Conjugated (bs-2178R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken, Rabbit.
How should bs-2178R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SM22 Alpha Polyclonal Antibody, RBITC Conjugated (bs-2178R-RBITC)?
KLH conjugated synthetic peptide derived from human SM22 Alpha
What are the excitation and emission wavelengths of the RBITC conjugate (bs-2178R-RBITC)?
RBITC on bs-2178R-RBITC: excitation / emission 540nm/625nm.