EDG8 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-21807R-Cy7-100uL |
| Product Name | EDG8 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Pig |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EDG8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 53637 |
| Swiss Prot | Q9H228 |
| Subcellular location | Cell membrane |
| Synonyms | EDG 8; Endothelial differentiation G protein coupled receptor 8; Endothelial differentiation G-protein-coupled receptor 8; Endothelial Differentiation Gene 8; Endothelial differentiation sphingolipid G protein coupled receptor 8; S1P receptor 5; S1P receptor Edg 8; S1P receptor Edg-8; S1P receptor Edg8; S1P5; S1PR5; S1PR5_HUMAN; Sphingosine 1 Phosphate Receptor 5; Sphingosine 1 phosphate receptor Edg 8; Sphingosine 1 phosphate receptor Edg8; Sphingosine 1-phosphate receptor 5; Sphingosine 1-phosphate receptor Edg-8; SPPR 1; SPPR 2; SPPR1; SPPR2. |
| Background | Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins (By similarity). May play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EDG8 Polyclonal Antibody, Cy7 Conjugated (bs-21807R-Cy7) validated for, and at what dilution?
bs-21807R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EDG8 Polyclonal Antibody, Cy7 Conjugated (bs-21807R-Cy7) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Pig.
How should bs-21807R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EDG8 Polyclonal Antibody, Cy7 Conjugated (bs-21807R-Cy7)?
KLH conjugated synthetic peptide derived from human EDG8
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-21807R-Cy7)?
Cy7 on bs-21807R-Cy7: excitation / emission 743nm/767nm.