MAP3K9 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-21910R-Cy5.5 |
| Product Name | MAP3K9 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MAP3K9 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4293 |
| Swiss Prot | P80192 |
| Subcellular location | Cytoplasm |
| Synonyms | MLK1; M3K9_HUMAN; Map3k9; MEKK9; Mitogen activated protein kinase kinase kinase 9; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1 (tyr and ser/thr specificity); Mixed lineage kinase 1; PRKE1. |
| Background | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MAP3K9 Polyclonal Antibody, Cy5.5 Conjugated (BS-21910R-CY5-5) validated for, and at what dilution?
BS-21910R-CY5-5 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAP3K9 Polyclonal Antibody, Cy5.5 Conjugated (BS-21910R-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Pig, Horse, Chicken, Rabbit.
How should BS-21910R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAP3K9 Polyclonal Antibody, Cy5.5 Conjugated (BS-21910R-CY5-5)?
KLH conjugated synthetic peptide derived from human MAP3K9
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-21910R-CY5-5)?
Cy5.5 on BS-21910R-CY5-5: excitation / emission 675nm/694nm.