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LBX1 Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-22016R-RBITC
Product Name LBX1 Polyclonal Antibody, RBITC Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat
Predicted Reactivity Human, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LBX1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 10660
Swiss Prot P52954
Subcellular location Nucleus
Synonyms Homeobox; HPX 6; HPX6 antibodyLady bird like homeobox; Ladybird homeobox 1; Ladybird homeobox homolog 1; Ladybird homeobox protein homolog 1; LBX 1; LBX 1H; LBX1H; Transcription factor LBX1; Transcription factor similar to D. melanogaster homeodomain protein lady bird late; LBX1_HUMAN.
Background This gene and the orthologous mouse gene were found by their homology to the Drosophila lady bird early and late homeobox genes. In the mouse, this gene is a key regulator of muscle precursor cell migration and is required for the acquisition of dorsal identities of forelimb muscles.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is LBX1 Polyclonal Antibody, RBITC Conjugated (bs-22016R-RBITC) validated for, and at what dilution?

bs-22016R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LBX1 Polyclonal Antibody, RBITC Conjugated (bs-22016R-RBITC) react with?

Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-22016R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LBX1 Polyclonal Antibody, RBITC Conjugated (bs-22016R-RBITC)?

KLH conjugated synthetic peptide derived from human LBX1

What are the excitation and emission wavelengths of the RBITC conjugate (bs-22016R-RBITC)?

RBITC on bs-22016R-RBITC: excitation / emission 540nm/625nm.