phospho-GEF H1 (Ser886) Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2201R-PE |
| Product Name | phospho-GEF H1 (Ser886) Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Arhgef2 around the phosphorylation site of Ser886 |
| Modification Site | Ser886 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9181 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | GEF H1phospho S886; Arhgef2Ser886; Lbcl1; Lfc; mKIAA0651; AA408978; ARHG2; ARHGEF 2; ARHGEF-2; ARHGEF2; GEF; GEF H1; GEF-H1; GEFH1; LFP40; MGC95068; P40; Prolerating cell nucleolar antigen p40; Protein GEF-H1; rho/rac guanine nucleotide exchange factor GEF 2; rho/rac guanine nucleotide exchange factor 2; rho/rac guanine nucleotide exchange factor. |
| Background | GEF-H1 is a Rho guanine nucleotide exchange factor that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (loss of interaction between GEF-H1 and microtubules leads to activation of Rho). This protein has also been shown to localize to tight junctions and modulate polarized cell permeability. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is phospho-GEF H1 (Ser886) Polyclonal Antibody, PE Conjugated (bs-2201R-PE) validated for, and at what dilution?
bs-2201R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-GEF H1 (Ser886) Polyclonal Antibody, PE Conjugated (bs-2201R-PE) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-2201R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-GEF H1 (Ser886) Polyclonal Antibody, PE Conjugated (bs-2201R-PE)?
KLH conjugated synthetic phosphopeptide derived from human Arhgef2 around the phosphorylation site of Ser886
What are the excitation and emission wavelengths of the PE conjugate (bs-2201R-PE)?
PE on bs-2201R-PE: excitation / emission 496,564nm/578nm.