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p45 NF-E2 Polyclonal Antibody

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Rabbit
Overview
Catalog # bs-22023R
Product Name p45 NF-E2 Polyclonal Antibody
Applications
WB ELISA IHC-P IHC-F IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NF-E2
Immunogen Range 273-373/373
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4778
Swiss Prot Q16621
Subcellular location Nucleus
Synonyms erythroid-derived 2 45 kDa subunit , Leucine zipper protein NF-E2 , Leucine zipper protein NFE2 , NF E2 , nfe2 , NFE2_HUMAN , Nuclear factor (erythroid derived 2), 45kDa , nuclear factor (erythroid-derived 2), 45kD , Nuclear factor , nuclear factor, erythroid 2 , Nuclear factor, erythroid derived 2 45 kDa subunit , p45 , p45 NF-E2 , p45 NFE2 , Transcription factor NF-E2 45 kDa subunit , Transcription factor NFE2 45 kDa subunit .
Background NFE2 (Nuclear Factor, Erythroid 2) is a Protein Coding gene. Diseases associated with NFE2 include Essential Thrombocythemia and Spherocytosis, Type 4. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Hematopoietic Stem Cell Differentiation. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and transcription coactivator activity. An important paralog of this gene is NFE2L1
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is p45 NF-E2 Polyclonal Antibody (bs-22023R) validated for, and at what dilution?

bs-22023R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200); ICC (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does p45 NF-E2 Polyclonal Antibody (bs-22023R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Rabbit.

How should bs-22023R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise p45 NF-E2 Polyclonal Antibody (bs-22023R)?

KLH conjugated synthetic peptide derived from human NF-E2 Immunogen range: 273-373/373.