p45 NF-E2 Polyclonal Antibody, Cy7 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-22023R-Cy7-100uL |
| Product Name | p45 NF-E2 Polyclonal Antibody, Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rabbit |
| Specifications | |
| Conjugation | Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NF-E2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4778 |
| Swiss Prot | Q16621 |
| Subcellular location | Nucleus |
| Synonyms | erythroid-derived 2 45 kDa subunit , Leucine zipper protein NF-E2 , Leucine zipper protein NFE2 , NF E2 , nfe2 , NFE2_HUMAN , Nuclear factor (erythroid derived 2), 45kDa , nuclear factor (erythroid-derived 2), 45kD , Nuclear factor , nuclear factor, erythroid 2 , Nuclear factor, erythroid derived 2 45 kDa subunit , p45 , p45 NF-E2 , p45 NFE2 , Transcription factor NF-E2 45 kDa subunit , Transcription factor NFE2 45 kDa subunit . |
| Background | NFE2 (Nuclear Factor, Erythroid 2) is a Protein Coding gene. Diseases associated with NFE2 include Essential Thrombocythemia and Spherocytosis, Type 4. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Hematopoietic Stem Cell Differentiation. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and transcription coactivator activity. An important paralog of this gene is NFE2L1 |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is p45 NF-E2 Polyclonal Antibody, Cy7 Conjugated (bs-22023R-Cy7) validated for, and at what dilution?
bs-22023R-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p45 NF-E2 Polyclonal Antibody, Cy7 Conjugated (bs-22023R-Cy7) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Rabbit.
How should bs-22023R-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p45 NF-E2 Polyclonal Antibody, Cy7 Conjugated (bs-22023R-Cy7)?
KLH conjugated synthetic peptide derived from human NF-E2
What are the excitation and emission wavelengths of the Cy7 conjugate (bs-22023R-Cy7)?
Cy7 on bs-22023R-Cy7: excitation / emission 743nm/767nm.