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p45 NF-E2 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • ELISA
  • IHC-P
  • IHC-F

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Rabbit
Overview
Catalog # bs-22023R-HRP
Product Name p45 NF-E2 Polyclonal Antibody, HRP Conjugated
Applications
WB ELISA IHC-P IHC-F
Reactivity Human
Predicted Reactivity Mouse, Rat, Rabbit
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NF-E2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 4778
Swiss Prot Q16621
Subcellular location Nucleus
Synonyms erythroid-derived 2 45 kDa subunit , Leucine zipper protein NF-E2 , Leucine zipper protein NFE2 , NF E2 , nfe2 , NFE2_HUMAN , Nuclear factor (erythroid derived 2), 45kDa , nuclear factor (erythroid-derived 2), 45kD , Nuclear factor , nuclear factor, erythroid 2 , Nuclear factor, erythroid derived 2 45 kDa subunit , p45 , p45 NF-E2 , p45 NFE2 , Transcription factor NF-E2 45 kDa subunit , Transcription factor NFE2 45 kDa subunit .
Background NFE2 (Nuclear Factor, Erythroid 2) is a Protein Coding gene. Diseases associated with NFE2 include Essential Thrombocythemia and Spherocytosis, Type 4. Among its related pathways are Response to elevated platelet cytosolic Ca2+ and Hematopoietic Stem Cell Differentiation. Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and transcription coactivator activity. An important paralog of this gene is NFE2L1
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500

Frequently asked questions

Which applications is p45 NF-E2 Polyclonal Antibody, HRP Conjugated (bs-22023R-HRP) validated for, and at what dilution?

bs-22023R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does p45 NF-E2 Polyclonal Antibody, HRP Conjugated (bs-22023R-HRP) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Rabbit.

How should bs-22023R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise p45 NF-E2 Polyclonal Antibody, HRP Conjugated (bs-22023R-HRP)?

KLH conjugated synthetic peptide derived from human NF-E2