PLK4/STK18 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-22111R-Biotin |
| Product Name | PLK4/STK18 Polyclonal Antibody, Biotin Conjugated |
| Applications |
IHC-P
IHC-F
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PLK4/STK18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 10733 |
| Swiss Prot | O00444 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | PLK 4; PLK-4; PLK4_HUMAN; Polo like Kinase 4; Polo-like kinase 4; Protein serine/threonine kinase; SAK; Serine/Threonine Kinase 18; Serine/threonine kinase; Serine/threonine protein kinase 18; Serine/threonine protein kinase PLK4; Serine/threonine protein kinase Sak; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase PLK4; Serine/threonine-protein kinase SAK; Snk Akin Kinase; STK 18. |
| Background | Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Its central role in centriole replication suggests a posible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2/CHK2. |
| Application Dilution | |
| IHC-P | 1:200-400 |
| IHC-F | |
Frequently asked questions
Which applications is PLK4/STK18 Polyclonal Antibody, Biotin Conjugated (bs-22111R-Biotin) validated for, and at what dilution?
bs-22111R-Biotin is validated for IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PLK4/STK18 Polyclonal Antibody, Biotin Conjugated (bs-22111R-Biotin) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Rabbit.
How should bs-22111R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PLK4/STK18 Polyclonal Antibody, Biotin Conjugated (bs-22111R-Biotin)?
KLH conjugated synthetic peptide derived from human PLK4/STK18