RIM2 Polyclonal Antibody

$Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RIM2 Polyclonal Antibody, Unconjugated (bs-22118R) at 1:200 overnight at 4\u00b0C, DAB staining.$

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with RIM2 Polyclonal Antibody, Unconjugated (bs-22118R) at 1:200 overnight at 4\u00b0C, DAB staining.

Applications

IHC-P
IHC-F
IF

Reactivity

Predicted Reactivity

Human
Mouse
Dog
Cow
Sheep
Pig
Horse
Chicken

Overview
Catalog #	bs-22118R
Product Name	RIM2 Polyclonal Antibody
Applications	IHC-P IHC-F IF
Reactivity	Rat
Predicted Reactivity	Human, Mouse, Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human RIM2
Immunogen Range	391-490/1411
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	9699
Swiss Prot	Q9UQ26
Subcellular location	Cell membrane
Synonyms	Non small cell lung cancer RimL3a protein; Rab3 interacting molecule 2; RAB3IP3; Regulating synaptic membrane exocytosis protein 2; Rims2; RIMS2_HUMAN.
Background	RIM2 is a rab effector involved in exocytosis. It may act as scaffold protein. It is thought to be an effector protein for Rab3, binding to Rab3 on synaptic vesicles in a GTP dependent manner.
Application Dilution
IHC-P	1:200-400
IHC-F
IF