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LPA1 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LPA1 Polyclonal Antibody, Unconjugated (bs-22137R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with LPA1 Polyclonal Antibody, Unconjugated (bs-22137R) at 1:200 overnight at 4\u00b0C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (LPA1) Polyclonal Antibody, Unconjugated (bs-22137R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (LPA1) Polyclonal Antibody, Unconjugated (bs-22137R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Bioss unconjugated antibody vial

Applications

  • IHC-P
  • IHC-F
  • IF

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Sheep
  • Pig
  • Rabbit
Overview
Catalog # bs-22137R
Product Name LPA1 Polyclonal Antibody
Applications
IHC-P IHC-F IF
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Sheep, Pig, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LPA1
Immunogen Range 1-50/364
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1902
Swiss Prot Q92633
Subcellular location Cytoplasm, Cell membrane
Synonyms EDG 2; Endothelial Cell Differentiation Gene-2; Endothelial differentiation gene 2; Endothelial Differentiation Lysophosphatidic Acid G Protein Coupled Receptor 2; G10 protein homolog; GPCR 26; GPCR26; Gpcr91; Kdt2; LPA 1; LPA receptor 1; LPA receptor EDG2; LPA1; LPAR1; Lysophosphatidic acid receptor 1; Lysophosphatidic acid receptor EDG2; MGC105279; MGC29102; LPAR1_HUMAN.
Background Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation.
Application Dilution
IHC-P 1:200-400
IHC-F
IF

Frequently asked questions

Which applications is LPA1 Polyclonal Antibody (bs-22137R) validated for, and at what dilution?

bs-22137R is validated for IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LPA1 Polyclonal Antibody (bs-22137R) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Rabbit.

How should bs-22137R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LPA1 Polyclonal Antibody (bs-22137R)?

KLH conjugated synthetic peptide derived from human LPA1 Immunogen range: 1-50/364.

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