IL-1RA Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2216R-Cy5 |
| Product Name | IL-1RA Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse IL-1RA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 16181 |
| Swiss Prot | P25085 |
| Subcellular location | Cytoplasm, Secreted |
| Synonyms | IL-1ra; F630041P17Rik; Interleukin-1 receptor antagonist protein; IL-1RN; IRAP; IL1 inhibitor; Il1rn |
| Background | Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is IL-1RA Polyclonal Antibody, Cy5 Conjugated (bs-2216R-Cy5) validated for, and at what dilution?
bs-2216R-Cy5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IL-1RA Polyclonal Antibody, Cy5 Conjugated (bs-2216R-Cy5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Pig.
How should bs-2216R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IL-1RA Polyclonal Antibody, Cy5 Conjugated (bs-2216R-Cy5)?
KLH conjugated synthetic peptide derived from mouse IL-1RA
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-2216R-Cy5)?
Cy5 on bs-2216R-Cy5: excitation / emission 625,650nm/670nm.