Integrin alpha 4 Polyclonal Antibody, APC-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-22181R-APC-Cy5.5-100uL |
| Product Name | Integrin alpha 4 Polyclonal Antibody, APC-Cy5.5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Specifications | |
| Conjugation | APC-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Integrin alpha 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3676 |
| Swiss Prot | P13612 |
| Synonyms | Alpha 4 subunit of VLA 4 receptor;ITG-_4: Antigen CD49D; CD 49d ; CD49d; CD49d antigen; CDw49d; IA4; Integrin alpha IV; ITGA 4; ITGA4; LPAM23; MGC90518; Very Late Activation Antigen 4; Very Late Activation Protein 4 Receptor Alpha 4 Subunit; VLA 4; VLA4; integrin alpha-4; ITA4_HUMAN; |
| Background | Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Integrin alpha 4 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-22181R-APC-CY5-5) validated for, and at what dilution?
BS-22181R-APC-CY5-5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Integrin alpha 4 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-22181R-APC-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.
How should BS-22181R-APC-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Integrin alpha 4 Polyclonal Antibody, APC-Cy5.5 Conjugated (BS-22181R-APC-CY5-5)?
KLH conjugated synthetic peptide derived from human Integrin alpha 4
What are the excitation and emission wavelengths of the APC-Cy5.5 conjugate (BS-22181R-APC-CY5-5)?
APC-Cy5.5 on BS-22181R-APC-CY5-5: excitation / emission 650nm/694nm.