phospho-Smad2 (Ser465) Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2224R-BF680 |
| Product Name | phospho-Smad2 (Ser465) Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Horse, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human Smad2 around Ser465 |
| Modification Site | Ser465 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4087 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Smad2Ser465; p-Smad2Ser465; Smad2S465; Mothers against decapentaplegic homolog 2; SMAD 2; Mothers against DPP homolog 2; Smad2; hMAD 2; hSMAD2; JV18 1; JV18; JV181; MAD; MAD Related Protein 2; MADH2; MADR2; MGC22139; MGC34440; Mothers Against Decapentaplegic Homolog 2; mothers against DPP homolog 2; SMAD 2; SMAD; SMAD2; SMAD2_HUMAN. |
| Background | SMAD2 or Mothers against decapentaplegic homolog 2 is a polypeptide that, as its name describes, is a homolog of the Drosophila gene: "Mothers against decepentaplegic". It belongs to the SMAD family of proteins, which belong to the TGF-Beta superfamily of modulators. Like many other TGF-Beta family members SMAD2 is involved in cell signalling. SMAD2 modulates signals of activin and TGF-Beta's. It interacts with SMAD anchor for receptor activation (SARA). The binding of ligands causes the phosphorylation of the SMAD2 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD2 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. Smad2 (Mothers against decapentaplegic homolog 2; SMAD 2; Mothers against DPP homolog 2;) |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Smad2 (Ser465) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2224R-BF680) validated for, and at what dilution?
bs-2224R-BF680 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Smad2 (Ser465) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2224R-BF680) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Horse, Chicken.
How should bs-2224R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Smad2 (Ser465) Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-2224R-BF680)?
KLH conjugated synthetic phosphopeptide derived from human Smad2 around Ser465
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-2224R-BF680)?
AbBy Fluor-680 on bs-2224R-BF680: excitation / emission 679nm/702nm.