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GASC1/KDM4C Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
Overview
Catalog # bs-22322R-Cy3-100uL
Product Name GASC1/KDM4C Polyclonal Antibody, Cy3 Conjugated
Applications
WB
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GASC1/KDM4C
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 23081
Swiss Prot Q9H3R0
Subcellular location Nucleus
Synonyms GASC 1 protein; GASC1 antibody Gene amplified in squamous cell carcinoma 1 protein; JHDM3C; JmjC domain containing histone demethylation protein 3C; Jumonji domain containing protein 2C; KDM4C_HUMAN.
Background Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate.
Application Dilution
WB 1:300-5000

Frequently asked questions

Which applications is GASC1/KDM4C Polyclonal Antibody, Cy3 Conjugated (bs-22322R-Cy3) validated for, and at what dilution?

bs-22322R-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GASC1/KDM4C Polyclonal Antibody, Cy3 Conjugated (bs-22322R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit.

How should bs-22322R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GASC1/KDM4C Polyclonal Antibody, Cy3 Conjugated (bs-22322R-Cy3)?

KLH conjugated synthetic peptide derived from human GASC1/KDM4C

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-22322R-Cy3)?

Cy3 on bs-22322R-Cy3: excitation / emission 512,550nm/570,615nm.