Phospho-TFEB(Ser142) Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-22337R-FITC-100uL |
| Product Name | Phospho-TFEB(Ser142) Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TFEB around the phosphorylation site of Ser142 |
| Modification Site | Ser142 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7942 |
| Swiss Prot | P19484 |
| Synonyms | TFEB(Phospho-Ser142); TFEB(Phospho-S142); TFEB(Phospho Ser142); p-TFEB(Ser142); Alpha TFEB; AlphaTFEB; bHLHe35; T cell transcription factor EB; T-cell transcription factor EB; TCFEB; Transcription factor EB; TFEB_HUMAN. |
| Background | Transcription factors of the basic helix-loop-helix zipper (bHLH-ZIP) family contain a basic domain, used for DNA binding, and HLH and ZIP domains, both used for oligomerization. TFEB was isolated from a human B-cell cDNA library using a binding sequence from the adenovirus major late promoter. This transcription factor probably binds to the USF/MLTF site and probably recognizes E-box sequences in the heavy-chain immunoglobulin enhancer. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Phospho-TFEB(Ser142) Polyclonal Antibody, FITC Conjugated (bs-22337R-FITC) validated for, and at what dilution?
bs-22337R-FITC is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-TFEB(Ser142) Polyclonal Antibody, FITC Conjugated (bs-22337R-FITC) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-22337R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-TFEB(Ser142) Polyclonal Antibody, FITC Conjugated (bs-22337R-FITC)?
KLH conjugated synthetic peptide derived from human TFEB around the phosphorylation site of Ser142
What are the excitation and emission wavelengths of the FITC conjugate (bs-22337R-FITC)?
FITC on bs-22337R-FITC: excitation / emission 494nm/518nm.