FGF2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-2235R-Cy5.5 |
| Product Name | FGF2 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Dog |
| Predicted Reactivity | Cow, Sheep, GuineaPig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | Full length of human bFGF Recombinded. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2247 |
| Swiss Prot | P09038 |
| Subcellular location | Nucleus, Secreted |
| Synonyms | Basic fibroblast growth factor; FGF basic; FGF-basic; BFGF; FGF-2; FGF B; FGF2; FGF2 basic; FGFB; Fibroblast growth factor 2 (basic); HBGF 2; HBGF-2; HBGF2; HBGH 2; HBGH2; Heparin binding growth factor 2 precursor; Prostatropin; FGF2_HUMAN. |
| Background | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is FGF2 Polyclonal Antibody, Cy5.5 Conjugated (BS-2235R-CY5-5) validated for, and at what dilution?
BS-2235R-CY5-5 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FGF2 Polyclonal Antibody, Cy5.5 Conjugated (BS-2235R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat, Dog. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep, GuineaPig.
How should BS-2235R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FGF2 Polyclonal Antibody, Cy5.5 Conjugated (BS-2235R-CY5-5)?
Full length of human bFGF Recombinded.
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-2235R-CY5-5)?
Cy5.5 on BS-2235R-CY5-5: excitation / emission 675nm/694nm.